Coding
Part:BBa_K641005
Designed by: Spencer Scott Group: iGEM11_Berkeley (2011-09-01)
ToxR with nhe1 and gly-ser linkers
This is the coding sequence for the ToxR protein that has an nhe1 restriction site before a double gly-ser linker that is used to connect many different types of dimerizing proteins to the N-terminal end of ToxR. This sequence will be the same for most ToxR systems unless changing the linker length. ToxR dimerizes when the protein attached to the N-terminus that sits in the periplasm dimerizes with another ToxR-dimerizing construct.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 637
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |