Coding

Part:BBa_K641005

Designed by: Spencer Scott   Group: iGEM11_Berkeley   (2011-09-01)


ToxR with nhe1 and gly-ser linkers

This is the coding sequence for the ToxR protein that has an nhe1 restriction site before a double gly-ser linker that is used to connect many different types of dimerizing proteins to the N-terminal end of ToxR. This sequence will be the same for most ToxR systems unless changing the linker length. ToxR dimerizes when the protein attached to the N-terminus that sits in the periplasm dimerizes with another ToxR-dimerizing construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 637
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None